![]() If small quantities of soil are used instead of roots, D. oviparasitica is rarely observed. Under these conditions, D. oviparasitica is probably not nutritionally dependent on nematode eggs, but it apparently grows saprophytically on roots and on cornmeal agar. The fungus often sporulates prolifically on roots, and occasionally sporulates on agar. Conidia do not always occur on repeated samples from the same root system, but the reasons for this variability are not known. Organisms colonize roots and agar, predacious fungi begin to decline, and conidia of D. oviparasitica can often be seen protruding from roots ( Mankau, 1975). Also, the disappearance of hyphae in eggs following destruction of the embryo sometimes makes it difficult to identify the original parasite. This method gives a direct indication of the number of eggs invaded by D. oviparasitica, but it does not differentiate between parasitism of viable eggs and saprobic growth in dead eggs. Inoculated media are examined daily for D. oviparasitica. Clumps of parasitized eggs were washed in sterile water and added to cornmeal agar (cornmeal infusion, 50 g agar, 15 g water, 1 L), glucose-peptone agar (glucose, 10 g peptone, 10 g agar, 16 g water, 1 L), or YPSS agar (yeast extract, 4 g K2HPO4, 1 g MgSO4–7H20, 0.5 g soluble starch, 20 g agar, 16 g water, 1 L). Stirling and Mankau (1978a) collected the gelatinous matrix of Meloidogyne egg masses from host plants in the field and partially dissolved by treating in 1% NaOC1 for about 2 min, and eggs were examined for parasitic fungi. By combining the ITS sequence with morphologic characteristics, a new anamorphic species was described and illustrated together with its teleomorph. Conidia were elongated ellipsoids, 1–2 septate, mostly 1 septate. Conidiophores were either not branched or occasionally branched, bearing divergent sterigmata on the tip with a single conidium on each. (2009) isolated D. alba, a new species from the ascospores of Orbilia alba collected in Wenshan County, Yunnan Province, China. Therefore, it is described as a new anamorphic species, D. coccinella. Comparison of its morphologic and biologic characters and analysis of sequence data of the rDNA in ITS region suggest that the fungus is a separate species. Simple conidiophore that bears a single, cylindrical, 1-7- septate conidium with a round distal end, a monoblastic conidiogenous cell, cylindrical, and absence of nematode-trapping capability distinguish this anamorphic fungus from other Dactylella species. coccinella from the teleomorphic fungus Orbilia coccinella and described it as a new anamorphic species. Sterling and Mankau (1978) isolated D. oviparasitica from root knot nematode egg masses ( Meloidogyne spp.). Sunitha, in Beneficial Microbes in Agro-Ecology, 2020 3 Isolation of Dactylella
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